Due to their role in DNA damage response, certain poly (ADP-ribose) polymerase (PARP) protein family members are promising therapeutic targets for cancer. To identify inhibitors, researchers employ various in vitro assays to test for PARP enzymatic activity. Quickly generating high-quality data for inhibitor testing can be challenging, but by using optimized assay components, scientists can achieve sensitive and reproducible experiments to analyze drug specificity, effectiveness, and mechanism of action.
Download this ebook from BPS Bioscience to learn about
- The basics of the PARP family and their role in cancer
- Various ways to measure PARP enzymatic activity
- How to optimize PARP assays