Natural killer (NK) cells rapidly identify and kill foreign, infected, and tumorigenic cells, making them promising candidates for cell therapy. However, compared to T cells, modifying primary NK cells is much more difficult. CRISPR can make the process smoother by making site-specific gene insertion more efficient. Indeed, scientists recently reported that electroporation with Cas9/ribonucleoprotein complexes followed by adeno-associated virus transduction resulted in efficient, stable, and functional editing of NK cells.
Download this application note from Thermo Fisher Scientific to discover a protocol for generating genetically modified NK cells, featuring upstream and downstream cell processing optimization and a combination of viral and nonviral payload delivery methods.